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Engineered nanostructures of antigen provide an effective means for regulating mast cell activation.

Authors
  • Deng, Zhao1
  • Weng, I-Chun
  • Li, Jie-Ren
  • Chen, Huan-Yuan
  • Liu, Fu-Tong
  • Liu, Gang-yu
  • 1 Department of Chemistry, University of California, Davis, California 95616, USA.
Type
Published Article
Journal
ACS Nano
Publisher
American Chemical Society
Publication Date
Nov 22, 2011
Volume
5
Issue
11
Pages
8672–8683
Identifiers
DOI: 10.1021/nn202510n
PMID: 21999491
Source
Medline
Language
English
License
Unknown

Abstract

Nanostructures containing 2,4-dinitrophenyl (DNP) as antigen were designed and produced to investigate antibody-mediated activation of mast cells. The design consists of nanogrids of DNP termini inlaid in alkanethiol self-assembled monolayers (SAMs). Using scanning probe-based nanografting, nanometer precision was attained for designed geometry, size, and periodicity. Rat basophilic leukemia (RBL) cells exhibited high sensitivity to the geometry and local environment of DNP presented on these nanostructures. The impact included cellular adherence, spreading, membrane morphology, cytoskeleton structure, and activation. The highest level of spreading and activation was induced by nanogrids of 17 nm line width and 40 nm periodicity, with DNP haptens 1.4 nm above the surroundings. The high efficacy is attributed to two main factors. First, DNP sites in the nanostructure are highly accessible by anti-DNP IgE during recognition. Second, the arrangement or geometry of DNP termini in nanostructures promotes clustering of FcεRI receptors that are prelinked to IgE. The clustering effectively initiates Lyn-mediated signaling cascades, ultimately leading to the degranulation of RBL cells. This work demonstrates an important concept: that nanostructures of ligands provide new and effective cues for directing cellular signaling processes. © 2011 American Chemical Society

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