Affordable Access

deepdyve-link
Publisher Website

Endothelium-dependent vasodilatory effect of Smilax china Linn. water extract via PI3K/Akt signaling.

Authors
  • Tettey, Clement Okraku1
  • Yang, In-Jun2
  • Shin, Heung-Mook2, 3
  • 1 Department of Biomedical Sciences, University of Health and Allied Sciences, Ho, Ghana. , (Ghana)
  • 2 Department of Physiology College of Korean Medicine, Dongguk University, Gyeongju, South Korea. , (North Korea)
  • 3 Korea Promotion Institute for Traditional Medicine Industry, Gyeongsan, South Korea. , (North Korea)
Type
Published Article
Journal
Archives of physiology and biochemistry
Publication Date
Jul 01, 2020
Volume
126
Issue
3
Pages
209–213
Identifiers
DOI: 10.1080/13813455.2018.1508237
PMID: 30269606
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Objective: The objective of this study was to investigate the pharmacological effect of Smilax china Linn. water extract (SCLWE) on vascular relaxation and its underlying biochemical mechanisms.Methodology: Isolated rat aortic rings were pre-constricted with phenylephrine (PE). This was followed by the cumulative addition of SCLWE. The effect of endothelial nitric oxide and PI3K/Akt on the SCLWE-induced vasodilation was investigated by the pretreatment of endothelium-intact aortic strips with or without NG-nitro-L-arginine methyl ester (L-NAME) or wortmanin before constriction with PE.Results: Treatment of PE (1 μM)-pre-contracted aortic strips with SCLWE induced endothelium-dependent relaxation, which was attenuated by L-NAME and wortmanin. Further studies using HUVECs indicated that nitrite production, eNOS and PI3K/PKB (Akt) phosphorylations were increased after exposure to SCLWE but was attenuated by pretreatment with wortmanin.Conclusion: These results suggest that SCLWE induces vasodilation by augmenting NO production in endothelial cells via PI3K/Akt-dependent eNOS phosphorylation.

Report this publication

Statistics

Seen <100 times