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Endothelin-1 as an autocrine/paracrine factor for human tumor cell lines.

Authors
Type
Published Article
Journal
Journal of Cardiovascular Pharmacology
0160-2446
Publisher
Ovid Technologies (Wolters Kluwer) - Lippincott Williams & Wilkins
Publication Date
Volume
17 Suppl 7
Identifiers
PMID: 1725438
Source
Medline
License
Unknown

Abstract

We determined whether endothelin-1 (ET-1) is produced and secreted by human tumor cell lines and whether ET-1 can affect cytosolic free Ca2+ concentrations ([Ca2+]i) in these cells. Dilution curves of extracts of cultured conditioned media from several human cancer cell lines (HeLa, HEp-2, DLD-1, and Ca9-22) were parallel to those of standard ET-1 in radioimmunoassay (RIA). Amounts of ET-1-like immunoreactivity released into conditioned media were reduced by omitting fetal bovine serum from culture media. Synthetic ET-1 dose-dependently induced gradual and sustained increases in [Ca2+]i in HeLa, HEp-2, GOTO, and Lovo cells, an effect completely abolished by a Ca2+ channel blocker or by chelating extracellular Ca2+. These data suggest an autocrine/paracrine role of ET-1 in human tumor cell lines.

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