Simple Summary Apparent digestibility of fat in diets does not reflect the true availability of fat, especially in low-fat diets. Estimation of endogenous losses of fat and fatty acids from the digestive tract is required for the determination of true digestibility of fat. This study evaluates the effect of oil sources on endogenous losses of fat and fatty acids in growing pigs in which endogenous losses were estimated by both regression and fat-free diet methods. Results indicate that the estimated values for endogenous losses of fat and fatty acids were not different in pigs fed palm oil, soybean oil, flaxseed oil or rapeseed oil. The fat-free diet had lower estimated values compared with the regression method. A fat-free diet method deserves to be explored further. These findings contribute to accurate estimation of endogenous losses of fat and fatty acids for vegetable oils in the future. Abstract An experiment was conducted to determine the effect of oil sources with differing degrees of fatty acid saturation on endogenous losses of fat (ELF) and fatty acids (ELFA) in growing pigs, in which endogenous losses were estimated by two methods. Sixty-eight growing barrows (initial body weight 31.13 ± 4.44 kg) were randomly allotted to a completely randomized design with 17 diets. Sixteen added-oil diets were formulated by adding four levels (2%, 4%, 6% and 8%) of palm oil (PO), soybean oil (SBO), flaxseed oil (FSO) and rapeseed oil (RSO) to a diet poor in fat, respectively. One fat-free diet was formulated from cornstarch, soy protein isolate and sucrose. All diets contained chromic dioxide (0.4%) as an indigestible marker. Results indicated that, according to the regression equations, the amounts of ELF in PO, SBO, FSO and RSO were 6.28, 5.30, 4.17 and 4.84 g/kg of dry matter intake (DMI), respectively. The true total tract digestibility of fat was greater ( p < 0.05) for FSO and RSO than for PO, and the ELFA were different from 0 only for C16:0, C18:0 and C18:1 in FSO, and C16:0 and C18:0 in RSO ( p < 0.05). The estimated values for ELF and ELFAs in pigs fed PO, SBO, FSO or RSO were not different. The amount of ELF determined by the fat-free diet method was 2.60 g/kg DMI, and the amounts of C16:0, C18:0, C18:1 and C18:2 in ELFAs were 0.28, 0.26, 0.03 and 0.02 g/kg DMI, respectively. The fat-free diet method had lower ELF and ELFA values compared with the regression method ( p < 0.01). Collectively, dietary vegetable oil sources do not affect estimation of ELF and ELFA, but different evaluation methods lead to varying estimates of endogenous losses in pigs.