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An EMS mutant library for carrot and genetic analysis of some mutants.

Authors
  • Wu, Zhe1
  • Liu, Zhenzhen1
  • Chang, Shuangfeng1
  • Zhao, Yuxuan1
  • 1 College of Horticulture, Shanxi Agricultural University, Taigu 030801, China. , (China)
Type
Published Article
Journal
Breeding science
Publication Date
Dec 01, 2020
Volume
70
Issue
5
Pages
540–546
Identifiers
DOI: 10.1270/jsbbs.20020
PMID: 33603549
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Carrot is among the top-ten most economically important vegetable crops in the world. However, the gene pool of cultivated carrot is narrow and the collection and preservation of carrot germplasm are very limited in China. In this study, seeds of carrot inbred line "17005" were treated by ethyl methyl sulfone (EMS) to construct a mutant library. The conditions of EMS mutagenesis on inbred line "17005" were optimized, including treatment of seeds at 0.5% EMS for 6 h. We obtained a number of mutant lines showing inheritable morphological mutations in cotyledon, growth point, leaf, taproot in the vegetative phase and mutations in bolting time, primary stem height and color, secondary branch, flower and seed in the reproductive phase through M2 and M3. The F2 segregating population from the cross of yellow taproot and purple-red epidermis taproot with wild type showed that these two mutants were controlled by single recessive gene respectively. The inheritable mutants can not only enrich the carrot germplasm, but also provide resources for the function research of carrot gene. Copyright © 2020 by JAPANESE SOCIETY OF BREEDING.

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