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Elucidation of cell killing mechanism by comparative analysis of photoreactions on different types of bacteria.

Authors
Type
Published Article
Journal
Photochemistry and Photobiology
0031-8655
Publisher
Wiley Blackwell (Blackwell Publishing)
Publication Date
Volume
88
Issue
2
Pages
414–422
Identifiers
DOI: 10.1111/j.1751-1097.2011.01057.x
PMID: 22145679
Source
Medline
License
Unknown

Abstract

The mechanism of biocidal action of nano titania on Escherichia coli and Staphylococcus aureus has been evaluated by various biochemical techniques like lipid peroxidation, hydrolysis of orthonitrophenol β-D-galactopyranoside, estimation of protein-amino acid and bacterial nucleic acids leakage into solution, in addition to morphology studies by electron microscopy (TEM and SEM) and K(+) ion leakage by inductively coupled plasma optical emission spectrometry. The active anatase phase of nano titania has been synthesized by sol-gel and pulverization techniques to obtain particle sizes averaging around 11 nm. The nano semiconductor with a bandgap of 3.2 eV responds well to the UV source to liberate reactive oxygen species (ROS). Gram negative bacteria easily succumb to the ROS at a faster rate than gram-positive bacteria with an observable difference in the mode of attack. The use of analytical techniques revealed the release of peroxidized lipid (26 nmol mL(-1) ) and protein content (370 μg mL(-1)) with a K(+) ion concentration of 22 000 ppb on complete destruction of E. coli.

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