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Elevated expression of microRNA-30b in osteoarthritis and its role in ERG regulation of chondrocyte.

Authors
  • Li, Lisong1
  • Yang, Cao2
  • Liu, Xianzhe2
  • Yang, Shuhua2
  • Ye, Shunan2
  • Jia, Jie2
  • Liu, Wei2
  • Zhang, Yukun3
  • 1 Department of Orthopedics, the First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China. , (China)
  • 2 Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. , (China)
  • 3 Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. Electronic address: [email protected]. , (China)
Type
Published Article
Journal
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
Publication Date
Dec 01, 2015
Volume
76
Pages
94–99
Identifiers
DOI: 10.1016/j.biopha.2015.10.014
PMID: 26653555
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

ERG (ETS-related gene) belongs to the ETS family of transcription factors, and has been recently reported to contribute to homeostatic balance in skeleton cell plasticity. MicroRNA-30 (miR-30) family is also demonstrated to play a role in controlling chondrocyte differentiation. The current study investigated the miR-30b and ERG expression in articular cartilage of osteoarthritis (OA) patients. A total of 20 subjects, with 10 OA patients and 10 healthy participants, were included in this study. Human chondrosarcoma cell line SW1353 was used to explore the relationship of miR-30b and ERG in vitro. In OA patients, a significant increase of miR-30b and a decrease of ERG were observed in articular cartilage compared with Normal ones. MiR-30b mimic down-regulated the ERG mRNA and protein expression levels, while miR-30b inhibitor up-regulated ERG expression. In addition, miR-30b mimic also decreased the mRNA expression of COL2a and aggrecan, while miR-30b inhibitor had the opposite effect. Luciferase reporter assay confirmed that miR-30b targeted ERG. In conclusion, miR-30b was involved in the process of OA, and it probably functioned through its target gene ERG. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

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