Affordable Access

Electron microscopy and image processing applied to the study of protein structure and protein-protein interactions.

Authors
Type
Published Article
Journal
Journal of Ultrastructure Research
0022-5320
Publisher
Elsevier
Publication Date
Volume
88
Issue
2
Pages
143–176
Identifiers
PMID: 6400029
Source
Medline
License
Unknown

Abstract

We review the application of electron microscopy and image processing at the molecular level to an ever increasing range of biological specimens. Although recent advances have been due in part to development of more sophisticated instrumentation and/or processing algorithms, widespread application of the well-known techniques of image enhancement and structure reconstruction has depended on new strategies of in vitro crystallization and polymerization, some of which are outlined here. We also discuss the use of stoichiometric labeling and/or "cocrystallization" in identifying the different subunits in multisubunit complexes and in studying protein-protein interactions.

Statistics

Seen <100 times