Affordable Access

deepdyve-link
Publisher Website

Electrokinetically operated microfluidic devices for integrated immunoaffinity monolith extraction and electrophoretic separation of preterm birth biomarkers.

Authors
  • Sonker, Mukul1
  • Parker, Ellen K
  • Nielsen, Anna V
  • Sahore, Vishal
  • Woolley, Adam T
  • 1 Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA. [email protected]
Type
Published Article
Journal
The Analyst
Publisher
The Royal Society of Chemistry
Publication Date
Dec 18, 2017
Volume
143
Issue
1
Pages
224–231
Identifiers
DOI: 10.1039/c7an01357d
PMID: 29136068
Source
Medline
License
Unknown

Abstract

Biomarkers are often present in complex biological fluids like blood, requiring multiple, slow sample preparation steps that pose limitations in simplifying analysis. Here we report integrated immunoaffinity extraction and separation devices for analysis of preterm birth biomarkers in a human blood serum matrix. A reactive polymer monolith was used for immobilization of antibodies for selective extraction of target preterm birth biomarkers. Microfluidic immunoaffinity extraction protocols were optimized and then integrated with microchip electrophoresis for separation. Using these integrated devices, a ∼30 min analysis was carried out on low nanomolar concentrations of two preterm birth biomarkers spiked in a human serum matrix. This work is a promising step towards the development of an automated, integrated platform for determination of preterm birth risk.

Report this publication

Statistics

Seen <100 times