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Electrochemiluminescence detection with integrated indium tin oxide electrode on electrophoretic microchip for direct bioanalysis of lincomycin in the urine.

Authors
  • Zhao, Xiaocui
  • You, Tianyan
  • Qiu, Haibo
  • Yan, Jilin
  • Yang, Xiurong
  • Wang, Erkang
Type
Published Article
Journal
Journal of Chromatography B
Publisher
Elsevier
Publication Date
Oct 15, 2004
Volume
810
Issue
1
Pages
137–142
Identifiers
PMID: 15358317
Source
Medline
License
Unknown

Abstract

In this article, an antibiotic, lincomycin was determined in the urine sample by microchip capillary electrophoresis (CE) with integrated indium tin oxide (ITO) working electrode based on electrochemiluminescence (ECL) detection. This microchip CE-ECL system can be used for the rapid analysis of lincomycin within 40s. Under the optimized conditions, the linear range was obtained from 5 to 100 microM with correlation coefficient of 0.998. The limit of detection (LOD) of 3.1 microM was obtained for lincomycin in the standard solution. We also applied this method to analyzing lincomycin in the urine matrix. The limit of detection of 9.0 microM was obtained. This method can determine lincomycin in the urine sample without pretreatment, which demonstrated that it is a promising method of detection of lincomycin in clinical and pharmaceutical area.

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