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Electrochemically induced oxidative damage to double-stranded calf thymus DNA adsorbed on gold electrodes.

Authors
  • Ferapontova, E E
  • Shipovskov, S V
Type
Published Article
Journal
Biochemistry. Biokhimii︠a︡
Publication Date
Jan 01, 2003
Volume
68
Issue
1
Pages
99–104
Identifiers
PMID: 12693982
Source
Medline
License
Unknown

Abstract

Electrochemically induced oxidative damage to DNA was studied with double-stranded calf thymus DNA immobilized directly on a gold electrode surface. Pre-polarization of the DNA-modified electrodes at +0.5 V versus Ag/AgCl reference electrode, in a free from DNA blank buffer solution, pH 7.4, allowed for subsequent detection of direct electrochemical oxidation of adsorbed on gold DNA, in the potential range from +0.7 to +0.8 V. The redox potential of the process corresponded to the potentials of the oxidation of guanine bases in DNA. It is shown that with increasing potential scan rate, v, the mechanism of electrochemical oxidation of DNA changes from the irreversible 4e(-) oxidative damage of DNA at low v to reversible 1e(-) oxidation at high v, keeping the electrochemical activity of the adsorbed DNA layer virtually the same.

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