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Electrochemical characterization of enzymatic activity of yeast cells entrapped in a poly(dimethylsiloxane) microwell on the basis of limited diffusion system.

Authors
  • Shiku, Hitoshi
  • Goto, Shun
  • Jung, Sungbong
  • Nagamine, Kuniaki
  • Koide, Masahiro
  • Itayama, Tomosato
  • Yasukawa, Tomoyuki
  • Matsue, Tomokazu
Type
Published Article
Journal
The Analyst
Publisher
The Royal Society of Chemistry
Publication Date
Jan 01, 2009
Volume
134
Issue
1
Pages
182–187
Identifiers
DOI: 10.1039/b808428a
PMID: 19082191
Source
Medline
License
Unknown

Abstract

A highly sensitive and quantitative analysis was performed using a poly(dimethylsiloxane) (PDMS) microwell array in a scanning electrochemical microscopy setup. A microelectrode with a relatively large seal radius was used to cover the top of the cylindrical PDMS microwell (96 pL). The voltammogram for 4 mM ferrocyanide resulted in a charge value of 38 nC, suggesting that almost 100% of the reductant in the microwell was converted to the oxidation current. When genetically modified yeast cells were entrapped in the microwell, the accumulation of p-aminophenol (PAP) produced by expressing beta-galactosidase (betaGAL) was successfully observed.

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