We have developed a biosensor for the detection of live/viable bacteria based on the response of the conductive polymer 4-(3-pyrrolyl) butyric acid to glucose-induced metabolites. The polymer was electrochemically deposited and then functionalized with lectin through EDC/NHS chemistry to capture cells near the sensor surface and introduce selectivity for analytes. The addition of glucose to a three-electrode electrochemical cell containing 10 mM phosphate buffer and the bacteria-immobilized sensor produced an increase in the potential. When the bound bacteria were treated with antibiotics, the addition of glucose produced a notably reduced signal exhibiting the sensor’s potential to screen for the most effective antibiotic treatment. This biosensor having real-time responses, minimal sample preparation, and the ability to screen antibiotics demonstrates the speed, ease, and suitability essential for application in point-of-care services. The detection range was determined to be 6.0 × 103–9.2 × 107 CFU/mL.