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Electrochemical Biosensor for Rapid Detection of Viable Bacteria and Antibiotic Screening

Authors
  • Saucedo, Nuvia Maria
  • Srinives, Sira
  • Mulchandani, Ashok
Type
Published Article
Journal
Journal of Analysis and Testing
Publisher
Springer Singapore
Publication Date
Jan 01, 2019
Volume
3
Issue
1
Pages
117–122
Identifiers
DOI: 10.1007/s41664-019-00091-2
Source
Springer Nature
Keywords
License
Yellow

Abstract

We have developed a biosensor for the detection of live/viable bacteria based on the response of the conductive polymer 4-(3-pyrrolyl) butyric acid to glucose-induced metabolites. The polymer was electrochemically deposited and then functionalized with lectin through EDC/NHS chemistry to capture cells near the sensor surface and introduce selectivity for analytes. The addition of glucose to a three-electrode electrochemical cell containing 10 mM phosphate buffer and the bacteria-immobilized sensor produced an increase in the potential. When the bound bacteria were treated with antibiotics, the addition of glucose produced a notably reduced signal exhibiting the sensor’s potential to screen for the most effective antibiotic treatment. This biosensor having real-time responses, minimal sample preparation, and the ability to screen antibiotics demonstrates the speed, ease, and suitability essential for application in point-of-care services. The detection range was determined to be 6.0 × 103–9.2 × 107 CFU/mL.

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