Affordable Access

deepdyve-link
Publisher Website

Electrical method to quantify nanoparticle interaction with lipid bilayers.

Authors
  • Carney, Randy P
  • Astier, Yann
  • Carney, Tamara M
  • Voïtchovsky, Kislon
  • Jacob Silva, Paulo H
  • Stellacci, Francesco
Type
Published Article
Journal
ACS Nano
Publisher
American Chemical Society
Publication Date
Feb 26, 2013
Volume
7
Issue
2
Pages
932–942
Identifiers
DOI: 10.1021/nn3036304
PMID: 23267695
Source
Medline
License
Unknown

Abstract

Understanding as well as rapidly screening the interaction of nanoparticles with cell membranes is of central importance for biological applications such as drug and gene delivery. Recently, we have shown that "striped" mixed-monolayer-coated gold nanoparticles spontaneously penetrate a variety of cell membranes through a passive pathway. Here, we report an electrical approach to screen and readily quantify the interaction between nanoparticles and bilayer lipid membranes. Membrane adsorption is monitored through the capacitive increase of suspended planar lipid membranes upon fusion with nanoparticles. We adopt a Langmuir isotherm model to characterize the adsorption of nanoparticles by bilayer lipid membranes and extract the partition coefficient, K, and the standard free energy gain by this spontaneous process, for a variety of sizes of cell-membrane-penetrating nanoparticles. We believe that the method presented here will be a useful qualitative and quantitative tool to determine nanoparticle interaction with lipid bilayers and consequently with cell membranes.

Report this publication

Statistics

Seen <100 times