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Electric fields guide migration of epidermal stem cells and promote skin wound healing.

Authors
  • Li, Li1
  • Gu, Wei
  • Du, Juan
  • Reid, Brian
  • Deng, Xianjian
  • Liu, Zhidai
  • Zong, Zhaowen
  • Wang, Haiyan
  • Yao, Bo
  • Yang, Ce
  • Yan, Jun
  • Zeng, Ling
  • Chalmers, Laura
  • Zhao, Min
  • Jiang, Jianxin
  • 1 State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, China. , (China)
Type
Published Article
Journal
Wound Repair and Regeneration
Publisher
Wiley (Blackwell Publishing)
Publication Date
Jan 01, 2012
Volume
20
Issue
6
Pages
840–851
Identifiers
DOI: 10.1111/j.1524-475X.2012.00829.x
PMID: 23082865
Source
Medline
Language
English
License
Unknown

Abstract

Migration of epidermal stem cells (EpSCs) into wounds may play an important role in wound healing. Endogenous electric fields (EFs) arise naturally at wounds. Consistent with previous reports, we measured outward electric currents at rat skin wounds using vibrating probes. Topical use of prostaglandin E2 significantly promoted wound healing. However, it is not known whether EpSCs respond to EFs. We first isolated and characterized EpSCs from rat skin. We then demonstrated that EpSCs isolated from the epidermis migrated directionally toward the cathode in EFs of 50-400 mV/mm. The directedness values increased in a dose- and time-dependent fashion. The migration speed of EpSCs was significantly increased in EFs. EFs induced asymmetric polymerization of intracellular F-actin and activation of the extracellular signal-regulated kinase 1/2 and phosphatidylinositol-3-kinase (PI3K)/protein kinase B pathways. Inhibition of epidermal growth factor receptor, extracellular signal-regulated kinase 1/2, or PI3K significantly inhibited the cathodal distribution of F-actin and the electrotactic response of EpSCs. These data for the first time show that EpSCs possess obvious electrotaxis, in which the epidermal growth factor receptor-mitogen activated protein kinase-PI3K pathways are involved. These data thus suggest a novel aspect of electric signaling in wound healing-to stimulate and guide migration of EpSCs and to regulate wound healing. © 2012 by the Wound Healing Society.

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