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Egg-white protein fractionation using new weak anion-exchange resins based on poly(glycidyl methacrylate-co-ethylendimethacrylate). Preparation and characterization.

Authors
  • Paredes, B1
  • González, S
  • Rendueles, M
  • Villa-García, M A
  • Díaz, J M
  • 1 Department of Chemical Engineering and Environmental Technology, Oviedo University, Oviedo, Spain. , (Spain)
Type
Published Article
Journal
Journal of chromatographic science
Publication Date
Jan 01, 2005
Volume
43
Issue
5
Pages
241–248
Identifiers
PMID: 15975242
Source
Medline
License
Unknown

Abstract

A new formulation for a poly(glycidyl methacrylate-co-ethylendimethacrylate)-based resin with a 11:9 proportion of monomer to crosslinker is developed and amino-functionalized in order to obtain new particulate materials suitable for egg-white protein fractionation. Functionalization is carried out using three different chemical reagents: diethylamine (DEA), DEA-tetrahydrofuran (THF) (1:1), and concentrated ammonia. The ammonia- and DEA-THF-treated polymers are used to fractionate egg-white proteins, in particular lysozyme and ovalbumin, by anion-exchange chromatography in packed column experiments, the latter resin showing better performances. Finally, both supports, working at semipreparative scale and step-gradient elution, separate pure ovalbumin with a yield of 83%.

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