Egg-white protein fractionation using new weak anion-exchange resins based on poly(glycidyl methacrylate-co-ethylendimethacrylate). Preparation and characterization.
Department of Chemical Engineering and Environmental Technology, Oviedo University, Oviedo, Spain.
- Published Article
Journal of chromatographic science
- Publication Date
Jan 01, 2005
A new formulation for a poly(glycidyl methacrylate-co-ethylendimethacrylate)-based resin with a 11:9 proportion of monomer to crosslinker is developed and amino-functionalized in order to obtain new particulate materials suitable for egg-white protein fractionation. Functionalization is carried out using three different chemical reagents: diethylamine (DEA), DEA-tetrahydrofuran (THF) (1:1), and concentrated ammonia. The ammonia- and DEA-THF-treated polymers are used to fractionate egg-white proteins, in particular lysozyme and ovalbumin, by anion-exchange chromatography in packed column experiments, the latter resin showing better performances. Finally, both supports, working at semipreparative scale and step-gradient elution, separate pure ovalbumin with a yield of 83%.
Report this publication
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
This record was last updated on 04/18/2018 and may not reflect the most current and accurate biomedical/scientific data available from NLM.
The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/15975242