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Efficient protection from methotrexate toxicity and selection of transduced human hematopoietic cells following gene transfer of dihydrofolate reductase mutants.

Authors
  • Meisel, Roland
  • Bardenheuer, Walter
  • Strehblow, Claudia
  • Sorg, Ursula Regina
  • Elmaagacli, Ahmet
  • Seeber, Siegfried
  • Flasshove, Michael
  • Moritz, Thomas
Type
Published Article
Journal
Experimental Hematology
Publisher
Elsevier
Publication Date
Dec 01, 2003
Volume
31
Issue
12
Pages
1215–1222
Identifiers
PMID: 14662327
Source
Medline
License
Unknown

Abstract

Gene transfer of DHFR using suitable retroviral backbones and DHFR mutants significantly increases MTX resistance of human CFU-C and allows efficient in vitro selection of transduced cells using a short-term selection procedure.

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