Affordable Access

Efficient expression of a protein coding gene under the control of an RNA polymerase I promoter.

Authors
  • Palmer, T D
  • Miller, A D
  • Reeder, R H
  • McStay, B
Type
Published Article
Journal
Nucleic acids research
Publication Date
Jul 25, 1993
Volume
21
Issue
15
Pages
3451–3457
Identifiers
PMID: 8393988
Source
Medline
License
Unknown

Abstract

In mammalian cells, RNA polymerase I transcripts are uncapped and retain a polyphosphate 5' terminus. It is probably for this reason that they are poorly translated as messenger RNA. We show in this report that insertion of an Internal Ribosome Entry Site (IRES) into the 5' leader of an RNA polymerase I transcript overcomes the block to translation, presumably by substituting for the 5' trimethyl G cap. Addition of an SV40 polyA addition signal also enhances protein production from the RNA polymerase I transcript. RNA Polymerase I driven expression vectors containing both elements produce protein at levels comparable to that produced from RNA polymerase II driven expression vectors which utilize a retroviral LTR. RNA Polymerase I driven expression vectors may have a variety of uses both for basic research and for practical expression of recombinant proteins.

Report this publication

Statistics

Seen <100 times