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Efficient detection of soft agar grown colonies using a tetrazolium salt.

Authors
  • Schaeffer, W I
  • Friend, K
Type
Published Article
Journal
Cancer Letters
Publisher
Elsevier
Publication Date
May 01, 1976
Volume
1
Issue
5
Pages
259–262
Identifiers
PMID: 65214
Source
Medline
Language
English
License
Unknown

Abstract

The use of 2-(p-iodophenyl)-3-(p-nitrophenul)-5-phenyl tetrazolium chloride at concentrations of from 0.25-1.0 mg/ml, when added to plates containing soft agar grown colonies, colors the cell clusters a deep brick red against a colorless background. This greatly facilitates the quantitation of such colonies since clusters of cells containing as few as 16-32 cells are visible macroscopically. Incubation for 20 h is optimal although coloration takes place as early as 6-8 h.

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