Efficient delivery of small interfering RNA for inhibition of IL-12p40 expression in vivo

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Efficient delivery of small interfering RNA for inhibition of IL-12p40 expression in vivo

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BioMed Central
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PMC
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  • Biology
  • Medicine
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Abstract

1476-9255-1-4.fm ral ss BioMed CentJournal of Inflammation Open AcceResearch Efficient delivery of small interfering RNA for inhibition of IL-12p40 expression in vivo Marion A Flynn†, David G Casey†, Stephen M Todryk and Bernard P Mahon* Address: Institute of Immunology, National University of Ireland, Maynooth, Co. Kildare, Ireland Email: Marion A Flynn - [email protected]; David G Casey - [email protected]; Stephen M Todryk - [email protected] medicine.oxford.ac.uk; Bernard P Mahon* - [email protected] * Corresponding author †Equal contributors Abstract Background: RNA interference is an evolutionary conserved immune response mechanism that can be used as a tool to provide novel insights into gene function and structure. The ability to efficiently deliver small interfering RNA to modulate gene expression in vivo may provide new therapeutic approaches to currently intractable diseases. Methods: In vitro, siRNA targeting IL-12p40 was delivered to the murine macrophage cell line (J774A.1) encapsulated in a liposome with an IL-12 inducing agent (LPS/IFN-γ) over a number of time points. Controls included a variety of non-target specific siRNA reagents. Supernatants were analyzed for cytokine production while the cells were removed for mRNA profiling. In vivo, siRNA-targeting IL-12p40 was delivered to the murine peritoneal cavity in a therapeutic fashion, after endotoxin (LPS) challenge. Cells from the peritoneal cavity were removed by lavage and analyzed by flow cytometry. Levels of IL-12 present in lavage and in serum were also examined by ELISA. Results: In this report, we show that IL-12p40 siRNA can specifically silence macrophage expression of IL-12p40 mRNA and IL-12p70 protein in vitro. We extend this finding to demonstrate that delivery of liposome encapsulated siRNA targeting IL-12p40 to the murine peritoneal cavity can modulate an inflammatory stimulus in vivo. Furthermore, specific siRNA can be used therapeutically after endotoxin challenge to reduce bo

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