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Efficacy of ultraviolet light-emitting diodes (UV-LED) at four different peak wavelengths against Cryptosporidium parvum oocysts by inactivation assay using immunodeficient mice.

Authors
  • Takahashi, Karin1
  • Matsubayashi, Makoto2
  • Ohashi, Yukio3
  • Naohara, Jun3
  • Urakami, Itsuo4
  • Sasai, Kazumi5
  • Kido, Yasutoshi6
  • Kaneko, Akira7
  • Teramoto, Isao6
  • 1 Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 598-8531, Japan. , (Japan)
  • 2 Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 598-8531, Japan; Department of Parasitology, Graduate School of Medicine, Osaka City University, Osaka 545-8585, Japan; Asian Health Science Research Institute, Osaka Prefecture University, Osaka 598-8531, Japan; Department of Veterinary Parasitology, Faculty of Veterinary Medicine, Airlangga University, Surabaya 60115, Indonesia.. Electronic address: [email protected] , (Indonesia)
  • 3 Department of Biomedical Engineering, Faculty of Engineering, Okayama University of Science, Okayama 700-0005, Japan. , (Japan)
  • 4 Chiyoda Kohan Co., Ltd., Tokyo 104-8115, Japan. , (Japan)
  • 5 Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 598-8531, Japan; Asian Health Science Research Institute, Osaka Prefecture University, Osaka 598-8531, Japan. , (Japan)
  • 6 Department of Parasitology, Graduate School of Medicine, Osaka City University, Osaka 545-8585, Japan. , (Japan)
  • 7 Department of Parasitology, Graduate School of Medicine, Osaka City University, Osaka 545-8585, Japan; Department of Parasitology and Research Centre for Infectious Disease Sciences, Graduate School of Medicine, Osaka City University, Osaka 545-8585, Japan. , (Japan)
Type
Published Article
Journal
Parasitology international
Publication Date
Aug 01, 2020
Volume
77
Pages
102108–102108
Identifiers
DOI: 10.1016/j.parint.2020.102108
PMID: 32224132
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

As an alternative to using ultraviolet (UV) lamps, which are made with mercury that is toxic to the environment and human health, UV light-emitting diodes (UV-LEDs) are expected to be effective for inactivating microorganisms in water. Although UV-LEDs have been reported to be effective against bacteria and viruses, the effectiveness of UV-LEDs against Cryptosporidium parasites has not been fully evaluated. As we report here, we have developed an in vivo quantitative inactivation assay for C. parvum oocysts using immunodeficient mice. Using the assay, we evaluated the effectiveness of treatment by UV lamp (254 nm) at approximately 1000 μJ/cm2 (for 3 s at a distance of 95 mm) compared to inactivation by commercially available UV-LEDs (with peak wavelengths of 268, 275, 284, and 289 nm). The shed patterns of oocysts after treatment with 284- and 289-nm wavelength UV-LEDs were significantly delayed compared to that after treatment with a UV lamp. These findings provide the first suggestion that UV-LEDs are effective against these parasites, as assessed using commercially available 350-mA UV-LEDs under conditions of fixed exposure distance and time. Copyright © 2020 Elsevier B.V. All rights reserved.

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