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Efficacy of three innovative bacterin vaccines against experimental infection with Mycoplasma hyopneumoniae

Authors
  • Matthijs, Anneleen Marguerite Filip1
  • Auray, Gaël2, 3
  • Boyen, Filip4
  • Schoos, Alexandra1
  • Michiels, Annelies1
  • García-Nicolás, Obdulio2, 3
  • Barut, Güliz Tuba2, 3
  • Barnier-Quer, Christophe5
  • Jakob, Virginie5
  • Collin, Nicolas5
  • Devriendt, Bert6
  • Summerfield, Artur2, 3
  • Haesebrouck, Freddy4
  • Maes, Dominiek1
  • 1 Ghent University, Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Salisburylaan 133, Merelbeke, 9820, Belgium , Merelbeke (Belgium)
  • 2 Institute of Virology and Immunology, Sensemattstrasse 293, Mittelhäusern, 3147, Switzerland , Mittelhäusern (Switzerland)
  • 3 University of Bern, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, Länggassstrasse 120, Bern, 3012, Switzerland , Bern (Switzerland)
  • 4 Ghent University, Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Salisburylaan 133, Merelbeke, 9820, Belgium , Merelbeke (Belgium)
  • 5 University of Lausanne, Vaccine Formulation Laboratory, Chemin des Boveresses 155, Epalinges, 1066, Switzerland , Epalinges (Switzerland)
  • 6 Ghent University, Laboratory of Veterinary Immunology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Salisburylaan 133, Merelbeke, 9820, Belgium , Merelbeke (Belgium)
Type
Published Article
Journal
Veterinary Research
Publisher
BioMed Central
Publication Date
Nov 08, 2019
Volume
50
Issue
1
Identifiers
DOI: 10.1186/s13567-019-0709-0
Source
Springer Nature
License
Green

Abstract

New vaccine formulations that include novel strains of Mycoplasma hyopneumoniae and innovative adjuvants designed to induce cellular immunity could improve vaccine efficacy against this pathogen. The aim of this experimental study was to assess the efficacy of three experimental bacterin formulations based on M. hyopneumoniae field strain F7.2C which were able to induce cellular immunity. The formulations included a cationic liposome formulation with the Mincle receptor ligand trehalose 6,6-dibehenate (Lipo_DDA:TDB), a squalene-in-water emulsion with Toll-like receptor (TLR) ligands targeting TLR1/2, TLR7/8 and TLR9 (SWE_TLR), and a poly(lactic-co-glycolic acid) micro-particle formulation with the same TLR ligands (PLGA_TLR). Four groups of 12 M. hyopneumoniae-free piglets were primo- (day (D) 0; 39 days of age) and booster vaccinated (D14) intramuscularly with either one of the three experimental bacterin formulations or PBS. The pigs were endotracheally inoculated with a highly and low virulent M. hyopneumoniae strain on D28 and D29, respectively, and euthanized on D56. The main efficacy parameters were: respiratory disease score (RDS; daily), macroscopic lung lesion score (D56) and log copies M. hyopneumoniae DNA determined with qPCR on bronchoalveolar lavage (BAL) fluid (D42, D56). All formulations were able to reduce clinical symptoms, lung lesions and the M. hyopneumoniae DNA load in the lung, with formulation SWE_TLR being the most effective (RDSD28–D56 −61.90%, macroscopic lung lesions −88.38%, M. hyopneumoniae DNA load in BAL fluid (D42) −67.28%). Further experiments raised under field conditions are needed to confirm these results and to assess the effect of the vaccines on performance parameters.

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