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Effects of unsaturated fatty acid supplementation on phospholipid and triacylglycerol biosynthesis in Saccharomyces cerevisiae.

Authors
  • Casey, W M
  • Rolph, C E
  • Tomeo, M E
  • Parks, L W
Type
Published Article
Journal
Biochemical and biophysical research communications
Publication Date
Jun 30, 1993
Volume
193
Issue
3
Pages
1297–1303
Identifiers
PMID: 8323549
Source
Medline
License
Unknown

Abstract

A fatty acid desaturase mutant was used to study the regulatory effects of unsaturated fatty acids on glycerolipid biosynthesis in yeast. Cells grown on palmitoleic acid (16:1) contain approximately twice the amount of phospholipids and triacylglycerols (per mg. dry weight) compared to those grown on oleic acid (18:1). The in vitro specific activity of glycerol-3-phosphate acyl transferase was two fold higher when palmitoyl-CoA was used as a substrate relative to oleoyl-CoA. In vivo methylation studies revealed that cells grown on palmitoleic acid produce 2.6 fold more phosphatidylcholine via the CDP-DAG (methylation) pathway than cells grown on oleic acid, although oleic acid facilitated the direct phosphorylation of exogenously supplied choline. These data indicate that unsaturated fatty acids may act as key regulatory molecules which influence the glycerolipid biosynthetic matrix in yeast.

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