Affordable Access

deepdyve-link
Publisher Website

The Effects of Trypsin Inhibitor on Insulin Secretion Using Rat Pancreas in an Organ Bath

Authors
  • MORITA, ASUKA1
  • OUCHI, MOTOSHI1
  • SATOH, KEITARO2
  • KOBAYASHI, SHUNSUKE3
  • TERADA, MISAO4
  • WAKASHIN, HIDEFUMI5
  • KON, HIROE6
  • HAYASHI, KEITARO1
  • ANZAI, NAOHIKO4, 7
  • SHIMIZU, AKIRA8
  • SUGIHARA, HITOSHI3
  • OBA, KENZO9
  • FUJITA, TOMOE1
  • 1 Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine, Tochigi, Japan
  • 2 Department of Pharmacology, Meikai University School of Dentistry, Saitama, Japan
  • 3 Department of Endocrinology, Diabetes and Metabolism, Graduate School of Medicine, Nippon Medical School, Tokyo, Japan
  • 4 Dokkyo Medical University School of Medicine, Tochigi, Japan
  • 5 Department of Regulatory Physiology, Dokkyo Medical University School of Medicine, Tochigi, Japan
  • 6 Research Center for Laboratory Animals, Dokkyo Medical University, Tochigi, Japan
  • 7 Department of Pharmacology, Chiba University Graduate School of Medicine, Chiba, Japan
  • 8 Department of Analytic Human Pathology, Nippon Medical School, Tokyo, Japan
  • 9 Kawaguchi Sakura Clinic, Saitama, Japan
Type
Published Article
Journal
In Vivo
Publisher
International Institute of Anticancer Research
Publication Date
Sep 03, 2021
Volume
35
Issue
5
Pages
2551–2558
Identifiers
DOI: 10.21873/invivo.12537
PMID: 34410942
PMCID: PMC8408692
Source
PubMed Central
Keywords
Disciplines
  • Research Article
License
Unknown

Abstract

Background/Aim: We developed an experimental method to reproduce insulin secretion from isolated rat pancreas preparations using an organ bath system. However, secretion of trypsin, another pancreatic enzyme, interferes with insulin production in such systems. We aimed to ascertain the minimum trypsin inhibitor (TI), dose for obtaining a sustained, stable rate of insulin secretion. Materials and Methods: The action of TI (1-10 μg/ml) on pancreatic preparations of male Wistar-Imamichi rats in organ bath experiments was assessed by measuring insulin, amylase, and trypsin activity. Results: The level of insulin outflow remained steady in the TI-treated samples, in contrast to that in the untreated control, where insulin secretion decreased over time. The level of amylase outflow did not change significantly. Trypsin activity was significantly lower in the TI-treated samples than in the control. Conclusion: Even low concentrations of TI can maintain insulin secretion by inhibiting trypsin activity in organ bath experiments.

Report this publication

Statistics

Seen <100 times