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Effects of transgenic overexpression of diapause hormone and diapause hormone receptor genes on non-diapause silkworm.

Authors
  • Gong, Chunying1
  • Zeng, Wenhui1
  • Zhang, Tianyang1
  • Liu, Rongpeng1
  • Ou, Yao2
  • Ai, Junwen3
  • Xiang, Zhonghuai1
  • Xu, Hanfu4, 5
  • 1 State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400715, China. , (China)
  • 2 College of Biotechnology, Southwest University, Chongqing, 400715, China. , (China)
  • 3 The Sericultural Research Institute of Hunan Province, Changsha, 410127, Hunan, China. , (China)
  • 4 State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400715, China. [email protected] , (China)
  • 5 College of Biotechnology, Southwest University, Chongqing, 400715, China. [email protected] , (China)
Type
Published Article
Journal
Transgenic Research
Publisher
Springer-Verlag
Publication Date
Dec 01, 2017
Volume
26
Issue
6
Pages
807–815
Identifiers
DOI: 10.1007/s11248-017-0045-y
PMID: 28952064
Source
Medline
Keywords
License
Unknown

Abstract

Diapause is a state of developmental arrest that is most often observed in arthropods, especially insects. The domesticated silkworm, Bombyx mori, is a typical insect that enters diapause at an early embryonic stage. Previous studies have revealed that the diapause hormone (DH) signaling molecules, especially the core members DH and DH receptor 1 (DHR1), are crucial for the determination of embryonic diapause in diapause silkworm strains. However, whether they function in non-diapause silkworm strains remains largely unknown. Here, we generated two transgenic lines overexpressing DH or DHR1 genes in a non-diapause silkworm strain, Nistari. Our results showed that developmental expression patterns of DH and DHR1 are quite similar in transgenic silkworms: both genes are highly expressed in the mid to late stages of pupae and are most highly expressed in day-6 pupae but are expressed at very low levels in other developmental stages. Moreover, the overexpression of DH or DHR1 can affect the expression of diapause-related genes but is not sufficient to induce embryonic diapause in their offspring. This study provides new insights into the function of DH and DHR1 in a non-diapause silkworm strain.

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