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Effects of tissue handling on RNA integrity and microarray measurements from resected breast cancers.

Authors
Type
Published Article
Journal
JNCI Journal of the National Cancer Institute
1460-2105
Publisher
Oxford University Press
Publication Date
Volume
103
Issue
24
Pages
1871–1883
Identifiers
DOI: 10.1093/jnci/djr438
PMID: 22034635
Source
Medline

Abstract

Sample preservation in RNAlater improves RNA yield and quality, whereas cold ischemia increases RNA fragmentation as measured by the 3'/5' expression ratio of control genes. However, expression levels of single genes and multigene signatures that are of diagnostic relevance in breast cancer were mostly unaffected by sample preservation method or prolonged cold ischemic duration.

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