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Effects of tissue handling on RNA integrity and microarray measurements from resected breast cancers.

Authors
  • Hatzis, Christos
  • Sun, Hongxia
  • Yao, Hui
  • Hubbard, Rebekah E
  • Meric-Bernstam, Funda
  • Babiera, Gildy V
  • Wu, Yun
  • Pusztai, Lajos
  • Symmans, W Fraser
Type
Published Article
Journal
JNCI Journal of the National Cancer Institute
Publisher
Oxford University Press
Publication Date
Dec 21, 2011
Volume
103
Issue
24
Pages
1871–1883
Identifiers
DOI: 10.1093/jnci/djr438
PMID: 22034635
Source
Medline
License
Unknown

Abstract

Sample preservation in RNAlater improves RNA yield and quality, whereas cold ischemia increases RNA fragmentation as measured by the 3'/5' expression ratio of control genes. However, expression levels of single genes and multigene signatures that are of diagnostic relevance in breast cancer were mostly unaffected by sample preservation method or prolonged cold ischemic duration.

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