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Effects of temperature and salinity on survival, growth and DNA methylation of juvenile Pacific abalone, Haliotis discus hannai Ino

Authors
  • Kong, Ning1, 2
  • Liu, Xiao1
  • Li, Junyuan1, 2
  • Mu, Wendan3
  • Lian, Jianwu3
  • Xue, Yanjie3
  • Li, Qi4
  • 1 Chinese Academy of Sciences, Institute of Oceanology, Qingdao, 266071, China , Qingdao (China)
  • 2 University of Chinese Academy of Sciences, Beijing, 100049, China , Beijing (China)
  • 3 Ningbo University, Ningbo, 315211, China , Ningbo (China)
  • 4 Ocean University of China, Qingdao, 266003, China , Qingdao (China)
Type
Published Article
Journal
Chinese Journal of Oceanology and Limnology
Publisher
Science Press
Publication Date
Apr 20, 2017
Volume
35
Issue
5
Pages
1248–1258
Identifiers
DOI: 10.1007/s00343-016-5185-z
Source
Springer Nature
Keywords
License
Yellow

Abstract

Temperature and salinity are two of the most potent abiotic factors influencing marine mollusks. In this study, we investigated the individual and combined effects of temperature and salinity on the survival and growth of juvenile Pacific abalone, Haliotis discus hannai Ino, and also examined the DNA methylation alteration that may underpin the phenotypic variation of abalone exposed to different rearing conditions. The single-factor data showed that the suitable ranges of temperature and salinity were 16–28°C at a constant salinity of 32, and 24–40 at a constant temperature of 20°C, respectively. The two-factor data indicated that both survival and growth were significantly affected by temperature, salinity and their interaction. The optimal temperature-salinity combination for juveniles was 23–25°C and 30–36. To explore environment-induced DNA methylation alteration, the methylation-sensitive amplified polymorphism (MSAP) technique was used to analyze the genomic methylation profiles of abalone reared in optimal and adverse conditions. Neither temperature nor salinity induced evident changes in the global methylation level, but 67 and 63 differentially methylated loci were identified in temperature and salinity treatments, respectively. The between-group eigen analysis also showed that both temperature and salinity could induce epigenetic differentiation in H. discus hannai Ino. The results of our study provide optimal rearing conditions for juvenile H. discus hannai Ino, and represent the first step toward revealing the epigenetic regulatory mechanism of abalone in response to thermal and salt stresses.

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