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Effects of Saccharomyces cerevisiae on alleviating cytotoxicity of porcine jejunal epithelia cells induced by deoxynivalenol

  • Liu, Yang1
  • Chang, Juan1
  • Wang, Ping1
  • Yin, Qing-qiang1
  • Huang, Wei-wei1
  • Liu, Chao-qi1
  • Bai, Xian-xiao2
  • Zhu, Qun3
  • Gao, Tian-zeng4
  • Zhou, Pu5
  • 1 Henan Agricultural University, College of Animal Science and Veterinary Medicine, Zhengzhou, 40002, China , Zhengzhou (China)
  • 2 Henan Academy of Agricultural Sciences, Institute of Animal Husbandry and Veterinary Science, Zhengzhou, 450002, China , Zhengzhou (China)
  • 3 Henan Delin Biological Product Co. Ltd., Xinxiang, 453000, China , Xinxiang (China)
  • 4 Henan Guangan Biotechnology Co., Ltd., Zhengzhou, 450001, China , Zhengzhou (China)
  • 5 Wugang Animal Product Quality Inspection Center, Wugang, 467000, China , Wugang (China)
Published Article
AMB Express
Springer Berlin Heidelberg
Publication Date
Sep 03, 2019
DOI: 10.1186/s13568-019-0863-9
Springer Nature


Deoxynivalenol (DON) is one of the mycotoxins most frequently encountering in cereal-based foods throughout the world. Saccharomyces cerevisiae was used to alleviate porcine jejunal epithelia cell (IPEC-J2) injury induced by DON in this study. The results indicated that cell viability and proliferation rates were significantly decreased when DON concentrations were increased from 0 to 64 µM after 24 h incubation (p < 0.05). The longer incubation time and higher DON concentrations would cause more serious effects on cell viability. S. cerevisiae could significantly degrade DON and decrease lactic dehydrogenase (LDH) release in the cells induced by DON (p < 0.05). DON (4 µM) could increase necrotic and apoptotic cell rates as well as decrease viable cell rates, compared with the control group (p < 0.05). However, S. cerevisiae addition in the DON group could decrease necrotic, late apoptotic and early apoptotic cell rates by 38.05%, 46.37% and 44.78% respectively, increase viable cell rates by 2.35%, compared with the single DON group (p < 0.05). In addition, S. cerevisiae addition could up-regulate mRNA abundances of IL-6, IL-8 and IL-10 in IPEC-J2 cells (p < 0.05), but down-regulate mRNA abundances of tight junction proteins (TJP-1) and occludin by 36.13% and 50.18% at 1 µM of DON (p < 0.05). It could be concluded that S. cerevisiae was able to alleviate IPEC-J2 cell damage exposed to DON.

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