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Effects of restraint stress on the regulation of hippocampal glutamate receptor and inflammation genes in female C57BL/6 and BALB/c mice.

Authors
  • McWhirt, Joshua1
  • Sathyanesan, Monica2
  • Sampath, Dayalan3
  • Newton, Samuel S2
  • 1 Division of Basic Biomedical Sciences, University of South Dakota, Vermillion, SD, 57069, USA.
  • 2 Division of Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota, Vermillion, SD 57069, Sioux Falls VA Healthcare System, Sioux Falls, SD, 57105, USA.
  • 3 Division of Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota, Vermillion, SD, 57069, USA.
Type
Published Article
Journal
Neurobiology of stress
Publication Date
Feb 01, 2019
Volume
10
Pages
100169–100169
Identifiers
DOI: 10.1016/j.ynstr.2019.100169
PMID: 31193545
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The two strains of inbred mice, BALB/c and C57BL/6, are widely used in pre-clinical psychiatry research due to their differences in stress susceptibility. Gene profiling studies in these strains have implicated the inflammation pathway as the main contributor to these differences. We focused our attention on female mice and tested their response to 5- or 10-day exposure to restraint stress. We examined the stress induced changes in the regulation of 11 inflammatory cytokine genes and 12 glutamate receptor genes in the hippocampus of female BALB/c and C57BL/6 mice using quantitative PCR. Elevated proinflammatory cytokine genes include Tumor Necrosis Factor alpha (TNFa), nuclear factor kappa-light-chain-enhancer of activated B cells (NFKB), Interleukin 1 alpha (IL1a), Interleukin 1 receptor (IL1R), Interleukin 10 receptor alpha subunit (IL10Ra), Interleukin 10 receptor beta subunit (IL10Rb), and tumor necrosis factor (TNF) super family members. Our results show that BALB/c and C57BL/6 mice differ in the genes induced in response to stress exposure and the level of gene regulation change. Our results show that the gene regulation in female BALB/c and C57BL/6 mice differs between strains in the genes regulated and the magnitude of the changes.

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