Effects of oviducal cells on the maintenance of motility and fertilizing capacity of fowl spermatozoa stored in a diffusion chamber.
- Published Article
- Publication Date
Nov 01, 1983
Oviducal cells were cultured on the inner or outer side of the membrane filter of a diffusion chamber, and attempts were made to maintain fowl spermatozoa at 41 C in vitro. When spermatozoa were stored along with cultured cells in the diffusion chamber, the high fertilizing capacity was retained for at least 3 days, regardless of whether the spermatozoa stored in the diffusion chamber were in direct contact with the cultured cells. In contrast, spermatozoa stored in the cell-free diffusion chamber or in a test tube showed a relatively low or no fertilizing capacity, respectively.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/6657571