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Effects of mutagens on somatic embryogenesis and plant regeneration in groundnut

Authors
  • Muthusamy, A.1, 2
  • Vasanth, K.1, 3
  • Sivasankari, D.1
  • Chandrasekar, B. R.1
  • Jayabalan, N.1
  • 1 Bharathidasan University, Tiruchirappalli, School of Life Sciences, Tamil Nadu, 620024, India , Tamil Nadu (India)
  • 2 Manipal University, Manipa, Manipal Life Sciences Centre, Karnataka, 576104, India , Karnataka (India)
  • 3 Stellenbosch University, Institute of Wine Biotechnology, 7602, South Africa , (South Africa)
Type
Published Article
Journal
Biologia Plantarum
Publisher
Kluwer Academic Publishers-Consultants Bureau
Publication Date
Sep 01, 2007
Volume
51
Issue
3
Pages
430–435
Identifiers
DOI: 10.1007/s10535-007-0092-y
Source
Springer Nature
Keywords
License
Yellow

Abstract

The embryogenic calli (EC) were obtained from hypocotyl explants of groundnut (Arachis hypogaea L.) cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) in combination with 0.5 mg dm−3 6-benzylaminopurine (BAP). The EC were exposed to γ-radiation (10–50 Gy) or treated with 1–5 mM of ethyl methane sulphonate (EMS) or sodium azide (SA). The mutated EC were subcultured on embryo induction medium containing 20 mg dm−3 2,4-D. Somatic embryos (SE) developed from these calli were transferred to MS medium supplemented with BAP (2.0 mg dm−3) and 0.5 mg dm−3 2,4-D for maturation. The well-developed embryos were cultured on germination medium consisting of MS salts with 2.0 mg dm−3 BAP and 0.25 mg dm−3 naphthaleneacetic acid (NAA). Well-developed plantlets were transferred for hardening and hardened plants produced normal flowers and set viable seeds. The fresh mass of the EC, mean number of SE per explant and regeneration percentage were higher at lower concentrations of mutagens (up to 30 Gy/3 mM). Some abnormalities in regenerated plants were observed, especially variations in leaf shape.

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