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Effects of moderate high temperature and UV-B on accumulation of withanolides and relative expression of the squalene synthase gene in Physalis peruviana.

Authors
  • Şahİn, Günce1
  • Tellİ, Murat1
  • ÜnlÜ, Ercan Selçuk2
  • Pehlİvan KarakaŞ, Fatma1
  • 1 Department of Biology, Faculty of Arts and Science, Bolu Abant İzzet Baysal University, Bolu Turkey. , (Turkey)
  • 2 Department of Chemisty, Faculty of Arts and Science, Bolu Abant İzzet Baysal University, Bolu Turkey. , (Turkey)
Type
Published Article
Journal
Turkish journal of biology = Turk biyoloji dergisi
Publication Date
Jan 01, 2020
Volume
44
Issue
5
Pages
295–303
Identifiers
DOI: 10.3906/biy-2002-69
PMID: 33110367
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Physalis peruviana L. (Cape gooseberry) is a source for a variety of phytocompounds such as withanolides, withanone, withaferin A, and withanolide A. These withanolides are high-value drug candidates due to their various pharmacological properties. To meet the increasing demands for these compounds, plant cell technology offers a reliable alternative. Exogenous addition of elicitors is considered the most effective strategy for enhanced production of secondary metabolites. In this study, we investigated changes in withanolide accumulation and characterized the gene expression level changes of squalene synthase enzyme in P. peruviana shoot cultures exposed to mild nonlethal heat stress (45°C for 2 and 5 h) and UV-B radiation (313 nm for 15 min and 3 h). We demonstrated significant changes in withanolide content with 7.86- and 12.5-fold increases for 2- and 5-hmild high-temperature exposure times, respectively. Exposure to UV-B also changed the withanolide content by 7.22- and 7-fold increases for 15 min and 3 h exposure times, respectively. The relative expression level of squalene synthase gene showed consistent results with1.80- and 10.13-fold increases in withanolide for 2- and 5-h mild high-temperature exposure times, and 1.34- and 2.01-fold increases with 15 min and 3 h UV-B exposure times, respectively. Copyright © 2020 The Author(s).

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