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Effects of a mixture of low doses of atrazine and benzo[a]pyrene on the rat seminiferous epithelium either during or after the establishment of the blood-testis barrier in the rat seminiferous tubule culture model.

Authors
  • Durand, Philippe1
  • Blondet, Antonine2
  • Martin, Guillaume3
  • Carette, Diane4
  • Pointis, Georges5
  • Perrard, Marie-Hélène6
  • 1 Kallistem SAS, VétagroSup, 1 Avenue Bourgelat, Marcy-l'Etoile 69280, France. Electronic address: [email protected] , (France)
  • 2 Kallistem SAS, VétagroSup, 1 Avenue Bourgelat, Marcy-l'Etoile 69280, France. Electronic address: [email protected] , (France)
  • 3 Kallistem SAS, VétagroSup, 1 Avenue Bourgelat, Marcy-l'Etoile 69280, France. Electronic address: [email protected] , (France)
  • 4 INSERM U 1065, 151 Route de Saint-Antoine, Nice 06200, France. Electronic address: [email protected] , (France)
  • 5 INSERM U 1065, 151 Route de Saint-Antoine, Nice 06200, France. , (France)
  • 6 INSERM U 1208, Institut Cellule Souche et Cerveau, 18 avenue du Doyen Lépine, Bron 69500, France. Electronic address: [email protected] , (France)
Type
Published Article
Journal
Toxicology in vitro : an international journal published in association with BIBRA
Publication Date
Feb 01, 2020
Volume
62
Pages
104699–104699
Identifiers
DOI: 10.1016/j.tiv.2019.104699
PMID: 31689476
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Atrazine (ATZ), a widely used agricultural pesticide and benzo[a]pyrene (BaP), a ubiquitous environmental human carcinogen can induce alterations of spermatogenesis. In the present study, we showed first that our seminiferous tubule culture model, in bicameral chambers, allowed the settlement of the blood-testis barrier (BTB) in 8-day-old male rat cultures and the differentiation of spermatogonia into round spermatids.The effect of a mixture of 1 μg/L of ATZ and 1 μg/L of BaP was then investigated either during or after the establishment of the BTB by using 8- or 20-22-day-old rats. Cultures were performed over a 3-week period. Our results show that claudin-11 and connexin 43 two proteins of the BTB, were impaired by the mixture which also reduced the number of round spermatids (the direct precursors of spermatozoa), by targeting the middle to late pachytene spermatocytes. These effects were observed in 8- and 20-22-day -old rat seminiferous tubule cultures. However, the decrease of the number of round spermatids was faster and more marked in the 8-day- than in the 20-22-day -old rat seminiferous tubule cultures. Our study emphasizes the possible influence of the age of an individual on the effect of (a) toxicant(s) on spermatogenesis. Copyright © 2019 Elsevier Ltd. All rights reserved.

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