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Effects of methyl farnesoate on Krüppel homolog 1 (Kr-h1) during vitellogenesis in the Chinese mitten crab (Eriocheir sinensis).

Authors
  • Li, Xilei1
  • Chen, Tiantian2
  • Jiang, Hucheng3
  • Huang, Jiawei2
  • Huang, Mengting2
  • Xu, Ruihan2
  • Xie, Qiming2
  • Zhu, Haojie2
  • Su, Shiping4
  • 1 College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China. Electronic address: [email protected] , (China)
  • 2 College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China. , (China)
  • 3 Freshwater Fisheries Research Institute of Jiangsu Province, China. , (China)
  • 4 College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China. Electronic address: [email protected] , (China)
Type
Published Article
Journal
Animal reproduction science
Publication Date
Nov 22, 2020
Volume
224
Pages
106653–106653
Identifiers
DOI: 10.1016/j.anireprosci.2020.106653
PMID: 33249353
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Methyl farnesoate (MF), a de-epoxidized form of juvenile hormone (JH) Ⅲ in insects, may regulate developmental processes such as reproduction and ovarian maturation in crustaceans. Krüppel homolog 1 (Kr-h1) is a target response gene for the methoprene-tolerant (Met) protein that is a component of the JH signaling pathway in insects. In the present study, Es-Kr-h1 was cloned from E. sinensis and characterized to ascertain whether JH/MF signaling in insects is conserved in crustaceans. The findings with molecular structure analysis indicated Es-Kr-h1 contains seven zinc finger motifs (Zn2-Zn8) commonly conserved in other crustaceans, but the Zn1 motif was not detected to be present. The PCR results indicated that relative abundance of Es-Kr-h1 mRNA transcript in the hepatopancreas was greatest in the Stage Ⅱ, followed by the Stage Ⅳ ovarian developmental categories. The relative abundance of Es-Kr-h1 mRNA transcript in vitro was greater after MF addition to the hepatopancreas, however, not the ovarian tissues. The results from in vivo and eyestalk ablation experiments indicated the relative abundance of Es-Kr-h1 mRNA transcript was greater after MF treatment and bilateral eyestalk removal in the hepatopancreas, however, not ovarian tissues. Notably, there were effects of MF on relative abundance of Es-Kr-h1 mRNA transcript pattern. The Es-Kr-h1 protein, therefore, may be involved in MF-mediated vitellogenesis resulting from the response to Es-Met in E. sinensis, and the JH/MF signaling pathway is potentially conserved in crustaceans. Copyright © 2020 Elsevier B.V. All rights reserved.

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