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The effects of metformin treatment on the ovaries and uterus of offspring.

Authors
  • Ayas, Bülent1
  • Kırmızıkan, Seda1
  • Kocaman, Adem1
  • Avcı, Bahattin2
  • 1 Faculty of Medicine, Department of Histology and Embryology, Ondokuz Mayıs University, Samsun, Turkey. , (Turkey)
  • 2 Faculty of Medicine, Department of Biochemistry, Ondokuz Mayıs University, Samsun, Turkey. , (Turkey)
Type
Published Article
Journal
Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology
Publication Date
Jul 01, 2021
Volume
37
Issue
7
Pages
624–628
Identifiers
DOI: 10.1080/09513590.2020.1819002
PMID: 32930027
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The aim of this study is to investigate the effects of metformin treatment at different dosage levels on the ovaries and uteruses of rat offspring in the course of the intrauterine period. Saline, metformin (100 mg/kg/day), and metformin (200 mg/kg/day) were administered via oral gavage between the 6th and 15th days of gestation to the 9 pregnant rats (n = 3/group). After birth, 5 female offspring were separated from each group and perfused on the 60th day of postnatal development. The cortex and medulla volumes of the ovaries, the thicknesses of epithelium and endometrium of the uteruses and the total oocyte number density were estimated. In addition, the estradiol levels in blood samples were measured by the ELISA method. There were no statistically significant differences among the groups regarding the number of oocytes, the volumes of ovarian cortex, medulla, primary and secondary follicles (p > .05). In comparison with the control group, the volume of the tertiary follicle, the thickness of the uterus epithelium, and the estradiol level were significantly decreased in Metformin 200 group (p < .05). The gestational exposure to high dose metformin may result in decreased estradiol production and subsequently decreased endometrial thickness of offspring rats.

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