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Effects of maternal inhalation of carbon black nanoparticles on reproductive and fertility parameters in a four-generation study of male mice

Authors
  • Skovmand, Astrid1, 2
  • Jensen, Alexander C. Ø.1
  • Maurice, Clotilde3
  • Marchetti, Francesco3
  • Lauvås, Anna J.2
  • Koponen, Ismo K.1
  • Jensen, Keld A.1
  • Goericke-Pesch, Sandra2, 4
  • Vogel, Ulla1, 5
  • Hougaard, Karin S.1, 6
  • 1 The National Research Centre for the Working Environment, Copenhagen Ø, Denmark , Copenhagen Ø (Denmark)
  • 2 University of Copenhagen, Department of Veterinary Clinical Sciences, Frederiksberg C, Denmark , Frederiksberg C (Denmark)
  • 3 Environmental Health Science Research Bureau, Health Canada, Ottawa, ON, Canada , Ottawa (Canada)
  • 4 University of Veterinary Medicine Hannover, Reproductive Unit of the Clinics – Clinic for Small Animals, Foundation, Hannover, Germany , Hannover (Germany)
  • 5 Technical University of Denmark, Department of Micro- and Nanotechnology, Lyngby, Denmark , Lyngby (Denmark)
  • 6 Institute of Public Health, University of Copenhagen, Copenhagen K, Denmark , Copenhagen K (Denmark)
Type
Published Article
Journal
Particle and Fibre Toxicology
Publisher
BioMed Central
Publication Date
Mar 18, 2019
Volume
16
Issue
1
Identifiers
DOI: 10.1186/s12989-019-0295-3
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundPrevious findings indicate that in utero exposure to nanoparticles may affect the reproductive system in male offspring. Effects such as decreased sperm counts and testicular structural changes in F1 males have been reported following maternal airway exposure to carbon black during gestation. In addition, a previous study in our laboratory suggested that the effects of in utero exposure of nanoparticles may span further than the first generation, as sperm content per gram of testis was significantly lowered in F2 males. In the present study we assessed male fertility parameters following in utero inhalation exposure to carbon black in four generations of mice.ResultsFilter measurements demonstrated that the time-mated females were exposed to a mean total suspended particle mass concentration of 4.79 ± 1.86 or 33.87 ± 14.77 mg/m3 for the low and high exposure, respectively. The control exposure was below the detection limit (LOD 0.08 mg/m3). Exposure did not affect gestation and litter parameters in any generation. No significant changes were observed in body and reproductive organ weights, epididymal sperm parameters, daily sperm production, plasma testosterone or fertility.ConclusionIn utero exposure to carbon black nanoparticles, at occupationally relevant exposure levels, via maternal whole body inhalation did not affect male-specific reproductive, fertility and litter parameters in four generations of mice.

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