The consumption of food with monounsaturated fatty acids has been associated with the improvement of the inflammation and oxidative stress in overweight individuals. In the present study, we evaluate the effect of high-oleic peanut intake within a hypocaloric diet on inflammatory and oxidative status markers in overweight men. Sixty-four overweight men (BMI 26-35 kg / m2, 18-50 years old) participated in this randomized, controlled study for 4-weeks, allocated into three groups: control CT, n = 22), conventional peanut (CVP, n = 21) and high-oleic peanuts (HOP, n = 21). They followed a hypocaloric diet (-250 kcal/d) with or without 56g of high-oleic or conventional peanuts. After the intervention, the inflammatory markers did not show significant changes in fasting concentrations or postprandial response among the experimental groups (p>0.05). The activity of oxidative status markers glutathione S-transferase (GST) and nitric oxide (NO) remained unchanged after the intervention. However, in the control group, malondialdehyde (MDA) showed lower concentration in comparison to the baseline (p=0,020) and among the groups (p=0,002). In this study, the daily intake of high-oleic peanut within a hypocaloric diet did not modify the inflammatory markers and oxidative status in overweight men. More studies are needed to better understand the effect of high-oleic peanut intake on health outcomes.