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Effects of glucose on collagen mRNA levels and collagen secretion in EAhy 926 endothelial cell line.

Authors
  • Kössi, J
  • Muona, P
  • Tuukkanen, J
  • Ylä-Outinen, H
  • Kalliomäki, M
  • Risteli, J
  • Oikarinen, A
  • Laato, M
  • Peltonen, J
Type
Published Article
Journal
Annales chirurgiae et gynaecologiae. Supplementum
Publication Date
Jan 01, 2001
Issue
215
Pages
39–44
Identifiers
PMID: 12016747
Source
Medline
License
Unknown

Abstract

Diabetes mellitus (DM) is a complex metabolic disease associated with increased accumulation of extracellular matrix by endothelial cells and contributing to vascular complications of long-standing diabetes. On the other hand, DM is also associated with decreased accumulation of extracellular matrix in granulation tissue, which is suggested to be a consequence of impaired angiogenesis. The role of hyperglycemia in these situations is not fully understood. We examined the effects of high glucose concentrations on the gene expression and secretion of various collagens in cultured EAhy 926 endothelial cells. EAhy 926 endothelial cells expressed alpha 1(I) collagen mRNA at a low level and small amount of the corresponding peptide was secreted from the cells; mRNA was not affected but peptide secretion was increased by elevated glucose concentration. mRNAs for type III and VI collagens were not detected in the endothelial cells Furthermore, high glucose concentration in long term had no morphological effects on cultured endothelial cells but increased the expression of type IV collagen, which could rather be beneficial for angiogenesis in a healing wound. Our results suggest that high glucose concentration per se may contribute to increased accumulation of extracellular matrix in blood vessels but probably is not responsible for decreased angiogenesis and granulation tissue formation in diabetic patients.

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