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Effects of Fibroblast Growth Factor-2 and Other Microsupplements on the Productivity of IgG- and IgA-Producing Cell Lines

Authors
  • Argentova, V. V.1
  • Aliev, T. K.2
  • Gasparyan, M. E.3
  • Dolgikh, D. A.1, 3
  • Kirpichnikov, M. P.1, 3
  • 1 Biology Department, Lomonosov Moscow State University, Moscow, 119991, Russia , Moscow (Russia)
  • 2 Chemistry Department, Lomonosov Moscow State University, Moscow, 119991, Russia , Moscow (Russia)
  • 3 Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia , Moscow (Russia)
Type
Published Article
Journal
Applied Biochemistry and Microbiology
Publisher
Pleiades Publishing
Publication Date
Dec 01, 2020
Volume
56
Issue
9
Pages
902–908
Identifiers
DOI: 10.1134/S0003683820090021
Source
Springer Nature
Keywords
License
Yellow

Abstract

Several serum-free media have been developed and are successfully used at present for the cultivation of various recombinant eukaryotic cell lines. Therapeutic recombinant IgA antibodies have recently been increasingly used along with numerous IgG1 isotype antibodies. Therefore, it became necessary to improve the growth characteristics, metabolism, and productivity of cell cultures producing these antibodies. The stimulating effect of a complex supplement containing Zn salts and FGF-2 on cell cultivation on basal media, DMEM and IMDM, was revealed in this work. The addition of dextran sulfate sodium salt and iron citrate to the basal medium was accompanied by improved productivity of stable IgG- and IgA-producing cell lines, as well as the homogeneity and density of cell cultures, which is most important in IgA-antibody production.

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