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Effects of Euphorbia umbellata extracts on complement activation and chemotaxis of neutrophils.

Authors
  • Latansio de Oliveira, Thais1
  • Fontana, Pâmela Dias2
  • Bavia, Lorena3
  • Cruz, Luiza Stolz4
  • Crisma, Amanda Rabello5
  • Sassaki, Guilherme Lanzi6
  • Alencar Menezes, Leociley Rocha7
  • Wang, Mei8
  • Beltrame, Flávio Luís9
  • Messias-Reason, Iara Jose10
  • 1 Laboratory of Molecular Immunopathology, Clinical Hospital, Federal University of Paraná, Curitiba, Brazil. Electronic address: [email protected] , (Brazil)
  • 2 Laboratory of Molecular Immunopathology, Clinical Hospital, Federal University of Paraná, Curitiba, Brazil. Electronic address: [email protected] , (Brazil)
  • 3 Laboratory of Molecular Immunopathology, Clinical Hospital, Federal University of Paraná, Curitiba, Brazil. Electronic address: [email protected] , (Brazil)
  • 4 Laboratory of Phytotherapy, Phytotherapy Technology and Chemistry of Natural Products, State University of Ponta Grossa, Ponta Grossa, Brazil. Electronic address: [email protected] , (Brazil)
  • 5 Laboratory of Hematology, Federal University of Paraná, Curitiba, Brazil. Electronic address: [email protected] , (Brazil)
  • 6 NMR Center, Federal University of Parana, Curitiba, Brazil. Electronic address: [email protected] , (Brazil)
  • 7 NMR Center, Federal University of Parana, Curitiba, Brazil. Electronic address: [email protected] , (Brazil)
  • 8 Natural Products Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, University, Mississippi, USA. Electronic address: [email protected]
  • 9 Laboratory of Phytotherapy, Phytotherapy Technology and Chemistry of Natural Products, State University of Ponta Grossa, Ponta Grossa, Brazil. Electronic address: [email protected] , (Brazil)
  • 10 Laboratory of Molecular Immunopathology, Clinical Hospital, Federal University of Paraná, Curitiba, Brazil. Electronic address: [email protected] , (Brazil)
Type
Published Article
Journal
Journal of ethnopharmacology
Publication Date
Sep 05, 2020
Volume
265
Pages
113348–113348
Identifiers
DOI: 10.1016/j.jep.2020.113348
PMID: 32896626
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The species Euphorbia umbellata (leitosinha) has been traditionally used for the treatment of inflammatory diseases and cancer. Evaluation the effect of E. umbellata latex extracts obtained with hexane, chloroform, ethyl acetate and methanol on the activation of the complement pathways and neutrophil chemotaxis. The latex was partitioned using Soxhlet apparatus and hexane, chloroform, ethyl acetate and methanol as solvents. The classical and alternative pathway activity were performed by hemolytic assays with sensitized sheep or rabbit erythrocytes, respectively; the lectin pathway activity was quantified by ELISA, through the measurement of C4 molecules and the chemotaxis of human neutrophils was performed using 1% casein as the chemotactic inducer and Boyden's chamber. GC-Q-ToF and NMR analyses were applied to evaluate the chemical composition of E. umbellata latex extracts. All E. umbellata latex extracts exhibited an inhibitory effect on the activation of the alternative pathway. Methanol and ethyl acetate extracts inhibited the classical pathway while chloroform extract activated this pathway. Ethyl acetate and hexane extracts inhibited lectin activation. All E. umbellata extracts inhibited casein-induced neutrophil chemotaxis. Terpenes and phenolic compounds have been suggested to be present in the E. umbellta latex extracts. The E. umbellata latex was able to modulate the functions of the immune system. Thus, it is possible to infer that the terpenes and phenolic compounds of the phytocomplex of E. umbellata latex can contribute for the activity on the complement pathways. Copyright © 2020 Elsevier B.V. All rights reserved.

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