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Effects of early-life FGF2 on ultrasonic vocalizations (USVs) and the mu-opioid receptor in male Sprague-Dawley rats selectively-bred for differences in their response to novelty.

Authors
  • Turner, Cortney A1
  • Hagenauer, Megan H2
  • Aurbach, Elyse L2
  • Maras, Pamela M2
  • Fournier, Chelsea L2
  • Blandino, Peter Jr2
  • Chauhan, Rikav B2
  • Panksepp, Jaak3
  • Watson, Stanley J Jr4
  • Akil, Huda4
  • 1 Molecular & Behavioral Neuroscience Institute, University of Michigan, Ann Arbor, MI, USA. Electronic address: [email protected]
  • 2 Molecular & Behavioral Neuroscience Institute, University of Michigan, Ann Arbor, MI, USA.
  • 3 Department of Integrative Physiology & Neuroscience, Washington State University, Pullman, WA, USA.
  • 4 Molecular & Behavioral Neuroscience Institute, University of Michigan, Ann Arbor, MI, USA; Department of Psychiatry, University of Michigan, Ann Arbor, MI, USA.
Type
Published Article
Journal
Brain research
Publication Date
Jul 15, 2019
Volume
1715
Pages
106–114
Identifiers
DOI: 10.1016/j.brainres.2019.03.011
PMID: 30880118
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

In previous studies, early-life fibroblast growth factor-2 (FGF2) administration conferred resilience to developing anxiety-like behavior in vulnerable animals in adulthood. To follow up on this work, we administered FGF2 the day after birth to animals that differ in emotional behavior and further explored its long-term effects on affective behavior and circuitry. Selectively-bred "high responder" rats (bHRs) exhibit low levels of anxiety-like and depression-like behavior, whereas selectively-bred "low responders" (bLRs) display high levels of anxiety-like and depression-like behavior. We found that early-life administration of FGF2 decreased negative affect in bLRs during the early post-natal period, as indexed by 40 kHz ultrasonic vocalizations (USVs) in response to a brief maternal separation on PND11. FGF2 also increased positive affect during the juvenile period, as measured by 50 kHz USVs in response to heterospecific hand play ("tickling") after weaning. In general, we found that bHRs produced more 50 kHz USVs than bLRs. In adulthood, we measured opioid ligand and receptor expression in brain regions implicated in USV production and affect regulation by mRNA in situ hybridization. Within multiple affective brain regions, bHRs had greater expression of the mu opioid receptor than bLRs. FGF2 increased mu opioid expression in bLRs. The bLRs had more kappa and less delta receptor expression than bHRs, and FGF2 increased prodynorphin in bLRs. Our results provide support for further investigations into the role of growth factors and endogenous opioids in the treatment of disorders characterized by altered affect, such as anxiety and depression. Published by Elsevier B.V.

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