Affordable Access

deepdyve-link
Publisher Website

Effects of docosahexaenoic acid on some megakaryocytic cell gene expression of some enzymes controlling prostanoid synthesis

Authors
  • Guillot, Nicolas
  • Debard, Cyrille
  • Calzada, Catherine
  • Vidal, Hubert
  • Lagarde, Michel
  • Véricel, Evelyne
Type
Published Article
Journal
Biochemical and Biophysical Research Communications
Publication Date
Jan 01, 2008
Volume
372
Issue
4
Pages
924–928
Identifiers
DOI: 10.1016/j.bbrc.2008.05.155
Source
Elsevier
Keywords
License
Unknown

Abstract

Beneficial effects of docosahexaenoic acid (DHA) intake in the prevention of cardiovascular diseases are known, and platelets play a crucial role in cardiovascular complications. However, high doses of DHA may increase lipid peroxidation and induce deleterious effects, notably in platelets. This led us to investigate the effect of DHA on gene expression of some enzymes controlling redox status and prostanoid formation in human megakaryoblastic cells (MEG-01 cell line). MEG-01 cells were incubated in presence of DHA (10 and 100 μmol/L) for 6 h. DHA enrichment up-regulated glutathione peroxidase-1 and thromboxane synthase mRNA. DHA increased gene catalase expression and up-regulated PPAR β/δ and PPAR γ mRNA in presence of high concentration of DHA. In conclusion, our results support an antioxidant mechanism of DHA. The effects of DHA on cellular redox status could, with others, provide an explanation for the beneficial influence of low consumption of DHA on cardiovascular events.

Report this publication

Statistics

Seen <100 times