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The effects of central asymmetry on the propagation of palindromic DNA in bacteriophage lambda are consistent with cruciform extrusion in vivo.

Authors
  • Chalker, A F
  • Okely, E A
  • Davison, A
  • Leach, D R
Type
Published Article
Journal
Genetics
Publication Date
Feb 01, 1993
Volume
133
Issue
2
Pages
143–148
Identifiers
PMID: 8436264
Source
Medline
License
Unknown

Abstract

The propagation of lambda phages carrying long perfect palindromes has been compared with that of phages carrying imperfect palindromes with small regions of central asymmetry. The perfect palindromes confer a more deleterious phenotype than those with central asymmetry and the severity of the phenotype declines with the length of asymmetry in the range from O to 27 base pairs. These results argue that a center-dependent reaction is involved in the phenotypic effects of palindromic DNA sequences, consistent with the idea that cruciform extrusion occurs in vivo.

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