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Effects of Cannabis Use on the Protein and Lipid Profile of Olfactory Neuroepithelium Cells from Schizophrenia Patients Studied by Synchrotron-Based FTIR Spectroscopy

Authors
  • Saladrigas-Manjón, Sergi1
  • Dučić, Tanja
  • Galindo, Liliana2, 3
  • Fernández-Avilés, Cristina1
  • Pérez, Víctor2, 4
  • de la Torre, Rafael1
  • Robledo, Patricia1
  • 1 (R.d.l.T.)
  • 2 (V.P.)
  • 3 Department of Psychiatry, University of Cambridge, Cambridge CB2 0SZ, UK
  • 4 CIBERSAM and Department of Psychiatry, University Autonoma of Barcelona, 08193 Barcelona, Spain
Type
Published Article
Journal
Biomolecules
Publisher
MDPI AG
Publication Date
Feb 19, 2020
Volume
10
Issue
2
Identifiers
DOI: 10.3390/biom10020329
PMID: 32092878
PMCID: PMC7072126
Source
PubMed Central
Keywords
License
Green

Abstract

Schizophrenia (SCZ) is a neurodevelopmental disorder with a high genetic component, but the presence of environmental stressors can be important for its onset and progression. Cannabis use can be a major risk factor for developing SCZ. However, despite the available data on the neurobiological underpinnings of SCZ, there is an important lack of studies in human neuronal tissue and living cells addressing the effects of cannabis in SCZ patients. In this study, we analysed the most relevant bio-macromolecular constituents in olfactory neuroepithelium (ON) cells of healthy controls non-cannabis users, healthy cannabis users, SCZ patients non-cannabis users, and SCZ patients cannabis users using Synchrotron Radiation-Fourier Transform Infrared (SR-FTIR) spectrometry and microscopy. Our results revealed that SCZ patients non-cannabis users, and healthy cannabis users exhibit similar alterations in the macromolecular profile of ON cells, including disruption in lipid composition, increased lipid membrane renewal rate and lipid peroxidation, altered proteins containing more β-sheet structures, and showed an increase in DNA and histone methylation. Notably, these alterations were not observed in SCZ patients who use cannabis regularly. These data suggest a differential effect of cannabis in healthy controls and in SCZ patients in terms of the macromolecular constituents of ON cells.

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