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Effects of cadmium on cation concentrations in sycamore cells (Acer pseudoplatanus L.): application of EDXRF.

Authors
  • Carvalho, M L
  • Silveira, L
  • Casimiro, A
Type
Published Article
Journal
Analytical and bioanalytical chemistry
Publication Date
Apr 01, 2002
Volume
373
Issue
8
Pages
827–829
Identifiers
PMID: 12194045
Source
Medline
License
Unknown

Abstract

Knowledge of the element content of biological systems is important in enabling understanding of uptake mechanisms and physiological response to stress conditions. In this work concentrations of mineral elements in sycamore (Acer pseudoplatanus L.) cells grown in the presence of cadmium have been analysed and compared with concentrations in control cells. Energy-dispersive X-ray fluorescence (EDXRF) was used to quantify the nutrients K, Ca, Mn, Fe, Cu, and Zn present in the cells. The reproducibility and accuracy of the technique were demonstrated by analysis of biological reference materials. Exposure of sycamore cells to cadmium had induced variation in the content of some elements. Mn, Cu, Zn, and, particularly, Fe concentrations in cells exposed to Cd were higher than those found in control cells. Ca is adsorbed on the cell walls and the concentration of K is not affected by the presence of Cd.

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