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Effects of arginine and trehalose on post-thawed bovine sperm quality.

Authors
  • Öztürk, Caner1
  • Güngör, Şükrü2
  • Ataman, Mehmet Bozkurt3
  • Bucak, Mustafa Numan3
  • Başpinar, Nuri4
  • Ili, Pınar5
  • Inanç, Muhammed Enes2
  • 1 Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Aksaray University , Aksaray , Turkey. , (Turkey)
  • 2 Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University , Burdur , Turkey. , (Turkey)
  • 3 Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Selcuk University , Konya , Turkey. , (Turkey)
  • 4 Department of Biochemistry, Faculty of Veterinary Medicine, Selcuk University , Konya , Turkey. , (Turkey)
  • 5 Department of Medical Services and Techniques/Medical Laboratory Techniques Pr, Denizli Health Services Vocational High School, Pamukkale University , Denizli , Turkey. , (Turkey)
Type
Published Article
Journal
Acta veterinaria Hungarica
Publication Date
Sep 01, 2017
Volume
65
Issue
3
Pages
429–439
Identifiers
DOI: 10.1556/004.2017.040
PMID: 28956482
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The present study was conducted to examine the protective role of arginine and trehalose on post-thaw bull sperm and oxidative stress parameters. Five ejaculates for each bull were used in the study. Each ejaculate, split into three equal aliquots and diluted at 37 °C with base extenders containing 2 mM arginine, 25 mM trehalose and no antioxidant (control) was cooled to 5 °C and then frozen. Frozen straws were thawed in a water bath for evaluation. Supplementation of the semen extender with arginine decreased the percentages of post-thawed subjective motility (29 ± 8.21%), CASA motility (12.2 ± 5.69%) and progressive motility (3.52 ± 2.13%), compared with the controls (43 ± 2.73%, 55.4 ± 6.78% and 33.48 ± 4.14%, respectively, P < 0.05). Supplementation of the semen extender with trehalose produced a higher mitochondrial activity and sperm viability (36.3 ± 3.99% and 44.1 ± 2.18%) compared with the control (13 ± 8.15 and 31.7 ± 3.94%, respectively, P < 0.05). It was established that trehalose (95.1%) and arginine (92.8%) protect DNA integrity compared to the control (90.4%) (P < 0.05). Trehalose supplementation in semen extenders provided great benefit in terms of viability, mitochondrial activity, and intact sperm DNA on frozen-thawed bull sperm.

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