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Effects of antiphagocytic agents on penetration of Eimeria magna sporozoites into cultured cells.

Authors
  • Jensen, J B
  • Edgar, S A
Type
Published Article
Journal
The Journal of parasitology
Publication Date
Apr 01, 1976
Volume
62
Issue
2
Pages
203–206
Identifiers
PMID: 1263027
Source
Medline
License
Unknown

Abstract

Madin-Darby Bovine Kidney cells were treated with sodium flouride, iodoacetate, and 2-deosyglucose, reagents that block glycolysis, and thus reduce phagocytosis. Sporozoites readily entered cells whose ATP stores were largely depleted. They also entered cells treated with colchicine, colcemid, and vinblastine. These latter agents did not inhibit sporozite motility after 6 hr incubation. Cytochalasin B prevented penetration of cells by inhibiting the motility of sporozoites. This effect was reversible. Warm sporozoites entered cold cells 4 times more radily than cold sporozoites into warm cells. The above findings suggest that phagocytosis is not the mechanism for entry of E. magna sporozoites into cultured cells, but that sporozoite motility is of primary importance.

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