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Effects of activating GABAB1 receptor on proliferation, migration, invasion and epithelial-mesenchymal transition of ovarian cancer cells

Authors
  • Gao, Jun1
  • Gao, Yao2
  • Lin, Shixin2
  • Zou, Xia1
  • Zhu, Yukai2
  • Chen, Xintong2
  • Wan, Hong1
  • Zhu, Hong1
  • 1 the Third Affiliated Hospital of Nanchang University, Xiangshanbei Road 128, Donghu District, Nanchang, Jiangxi, 330008, China , Nanchang (China)
  • 2 Jiangxi Medical College of Nanchang University, Nanchang, Jiangxi, 330008, China , Nanchang (China)
Type
Published Article
Journal
Journal of Ovarian Research
Publisher
BioMed Central
Publication Date
Oct 24, 2020
Volume
13
Issue
1
Identifiers
DOI: 10.1186/s13048-020-00726-4
Source
Springer Nature
Keywords
License
Green

Abstract

ObjectiveThis study aimed to explore the effects of activating GABAB1 receptor by baclofen on proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of ovarian cancer cells.ResultsOne hundred μmol/L, 200 μmol/L and 300 μmol/L were selected as low, medium and high baclofen concentrations respectively. Cells were divided into four groups: Control, 100 μmol/L, 200 μmol/L and 300 μmol/L. Compared with the control group, the viability, colony formation, migration and invasion of SKOV3 cells were inhibited, and the apoptosis of SKOV3 cells were enhanced significantly at 200 μmol/L and 300 μmol/L baclofen. Moreover, they changed significantly with the increase of baclofen concentration. Compared with the control group, the expression of E-cadherin and GABAB1 increased and the N-cadherin expression decreased significantly in 200 μmol/L and 300 μmol/L groups. Higher concentration of baclofen induced higher expression of E-cadherin and lower expression of N-cadherin.ConclusionBaclofen inhibited the proliferation, cloning, migration, invasion and EMT of ovarian cancer cells by activating GABAB1 receptor. These results might contribute a lot to clarify the role and possible mechanism of GABAB1 receptor in ovarian cancer.

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