Affordable Access

Effects of ACTH 1-24 on hydrolysis of beta-casomorphin-4-nitroanilide in brain homogenates in vivo and in vitro.

Authors
  • Endröczi, E
  • Sasváry, M
  • Walentin, S
  • Simon, J
Type
Published Article
Journal
Acta physiologica Hungarica
Publication Date
Jan 01, 1994
Volume
82
Issue
2
Pages
131–138
Identifiers
PMID: 7887173
Source
Medline
License
Unknown

Abstract

Administration of ACTH 1-24 to 3-10 days old rats produced a significant decrease in hydrolysis of beta-casomorphin-4-nitroanilide (beta-CM-4NA) in the cytosolic fraction of brain homogenate in the first three hours after injection. Corticosterone treatment did not modify the hydrolysis of the substrate. ACTH 1-24 but not ACTH 4-10, Met-enkephalin or Leuenkephalin given to the brain homogenate resulted in a dose-dependent decrease in liberation of 4NA from beta-CM-4NA. Kinetic data suggest competitive inhibition of ACTH molecule on hydrolysis of beta-CMA-4NA. The ACTH treatment, however, did not influence the hydrolysis of Pro-Gly-4NA or Pro-Pro-4NA in the brain homogenate in vitro.

Report this publication

Statistics

Seen <100 times