Affordable Access

Effective quenching of fragment formation in negative ion oligonucleotide matrix-assisted laser desorption/ionization mass spectrometry through sodium adduct formation.

Authors
  • Stano, Michal
  • Flosadottir, Helga D
  • Ingolfsson, Oddur
Type
Published Article
Journal
Rapid communications in mass spectrometry : RCM
Publication Date
Jan 01, 2006
Volume
20
Issue
23
Pages
3498–3502
Identifiers
PMID: 17072905
Source
Medline
License
Unknown

Abstract

Metastable decay of the sodium-free and the sodium adducts of the negatively charged hexameric nucleotides 5'-d(TTXYTT)-3' (X and Y are dC, dG or dA) in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF-MS) is reported. We show that the sodium adduct formation may be used to quantitatively quench dissociation channels in oligonucleotide MALDI-MS, and we argue, that by means of effective steps in the sample preparation that lead to per-sodiation of the sample, the sample preparation and the mass resolution of oligonucleotide mass spectra in MALDI may be improved substantially. The dependency of the fragment formation on the degree of sodiation is also discussed in context with the underlying fragmentation mechanisms.

Report this publication

Statistics

Seen <100 times